complex v activity assay kit #701000  (Cayman Chemical)

Journal: Journal of medicinal chemistry

Article Title: New Mandelalides Expand a Macrolide Series of Mitochondrial Inhibitors

doi: 10.1021/acs.jmedchem.7b00990

Figure Lengend Snippet: The % rate of ATP-dependent NADH oxidation relative to control was calculated from a series of time-dependent reactions carried out in the presence of increasing concentrations of synthetic 1 using isolated mitochondria from bovine heart as the source of complex V ATP synthase activity. Complex V activity was determined for each reaction by measuring NADH absorbance at 340 nm over time (captured every 30 s for 30 min) at 25°C. The reaction rate was calculated from the slope of each curve and plotted as % activity relative to vehicle control (0.2 % DMSO). Single saturating concentrations of apoptolidin A (1 µM) and oligomycin A (12.5 µM) were used as control inhibitors of complex V. Graph shows a representative titration that was repeated twice with the same result.

Article Snippet: Complex V activity assay Mitochondrial ATPase activity was measured with a MitoCheck® Complex V Activity Assay kit (#701000) from Cayman Chemical (Ann Arbor, MI) according to the manufacturers instructions.

Techniques: Isolation, Activity Assay, Titration